Journal: Scientific Reports

Article Title: Effect of the type of reducing agents of silver ions in interpolyelectrolyte-metal complexes on the structure, morphology and properties of silver-containing nanocomposites

doi: 10.1038/s41598-020-64079-0

Figure Lengend Snippet: Images of antimicrobial test results of agar plates Ag-containing nanocomposites, obtained via chemical reduction of Ag + ions in the polymer films against S. aureus ( a ) and E. coli ( b ).

Article Snippet: The antimicrobial activity of IPEC–Ag nanocomposites, prepared by chemical reduction of Ag + ions in IMC was investigated using reference strains of opportunistic bacteria Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 35218 (as a model gram-positive and gram-negative bacteriа) .

Techniques: Polymer

Journal: Antibiotics (Basel, Switzerland)

Article Title: Size-Controlled Ammonium-Based Homopolymers as Broad-Spectrum Antibacterials.

doi: 10.3390/antibiotics12081320

Figure Lengend Snippet: Figure 2. Fluorescent microscopy images of E. coli stained using a live/dead assay (live are shown in green (SYTO 9) and dead are shown in red (PI)). Control bacteria (a–c) and bacteria following treatment with the three lead polymers (PDADMAC (d–f); PMETACL (g–i) and PVMBT (j–l)). Bacteria were imaged on a Zeiss Axiovert 200M, with a 40× objective, in the FITC channel (λex 488 nm) (a,d,g,i) and the Texas red channel (λex 561 nm) (b,e,f,j) with the merged images shown (c,f,h,k). Red fluorescence indicates loss of membrane integrity and signifies the bactericidal action of the compound (scale bar: 20 µm).

Article Snippet: Escherichia coli (DH5α), Salmonella typhimurium SLI344, Micrococcus luteus (ATCC 4698), and Bacillus subtilis (ATCC 6051) were used as Gram-positive and Gram-negative test strains.

Techniques: Microscopy, Staining, Live Dead Assay, Control, Bacteria, Membrane

Journal: Antibiotics (Basel, Switzerland)

Article Title: Size-Controlled Ammonium-Based Homopolymers as Broad-Spectrum Antibacterials.

doi: 10.3390/antibiotics12081320

Figure Lengend Snippet: Figure 3. SEM images of (a) untreated E. coli; (c) PDADMAC-20-treated E. coli; (d) PVMBT-40- treated E. coli; and (e) PMETACL-40-treated E. coli. (b) Untreated M. luteus; (f) PDADMAC-20-treated M. luteus; (g) PVMBT-40-treated M. luteus; and (h) PMETACL-40-treated M. luteus. Green arrows indicate bacteria with impaired membranes. Red arrows indicate the pores on the bacterial surface of M. luteus. Scale bars: 500 nm.

Article Snippet: Escherichia coli (DH5α), Salmonella typhimurium SLI344, Micrococcus luteus (ATCC 4698), and Bacillus subtilis (ATCC 6051) were used as Gram-positive and Gram-negative test strains.

Techniques: Bacteria

Journal: Antibiotics (Basel, Switzerland)

Article Title: Size-Controlled Ammonium-Based Homopolymers as Broad-Spectrum Antibacterials.

doi: 10.3390/antibiotics12081320

Figure Lengend Snippet: Figure 4. Permeabilization of the outer membrane of E. coli was measured using a 1-N- phenyl-naphthylamine (NPN) assay in the presence of polymers PVMBT-40, PDADMAC-20, and PMETACL-40 after 20 min. Bacteria were incubated with NPN for 30 min to stabilize the fluorescence (data are not shown) and polymers added at 4× MIC (3.6 µM). Bacteria without polymer treatment were used as a negative control, while Triton X-100 (1% v/v)-treated bacteria served as the positive control (n = 3).

Article Snippet: Escherichia coli (DH5α), Salmonella typhimurium SLI344, Micrococcus luteus (ATCC 4698), and Bacillus subtilis (ATCC 6051) were used as Gram-positive and Gram-negative test strains.

Techniques: Membrane, NPN Assay, Bacteria, Incubation, Polymer, Negative Control, Positive Control

Journal: Cell Reports

Article Title: Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CL pro substrate degradome

doi: 10.1016/j.celrep.2021.109892

Figure Lengend Snippet:

Article Snippet: The primary antibodies and dilutions used were: mouse monoclonal anti-SARS-CoV-2 nucleocapsid antibody (1:1,000, Invitrogen, MA5-29981, RRID: AB_2785780 ); rabbit anti-SARS-CoV-1 3CL pro antibody (1:2000, Rockland, 200-401-A51, RRID: AB_828457 ); rabbit polyclonal anti-RPAP1 antibody (1:1,000, Proteintech, 15138-1-AP, RRID: AB_2301137 ); mouse monoclonal anti-PTBP1 antibody (1:500, Biolegend, 630101, 3H7, RRID: AB_2171285 ); rabbit polyclonal anti-MAP4K5 antibody (1:1,000, Cusabio, CSB-PA013440DSR2HU, RRID: AB_2892084 ); rabbit polyclonal anti-CREB1 antibody (1:1,000, Abclonal, A11989, RRID: AB_2758916 ); rabbit polyclonal anti-YAP1 antibody (1:1,000, Abclonal, A11430, RRID: AB_2758556 ); rabbit polyclonal anti-FYCO1 antibody (1:1,000, Cusabio, CSB-PA866262LA01HU, RRID: AB_2892085 ); rabbit polyclonal anti-FAF1 antibody (1:1,000, Abclonal, A2921, RRID: AB_2764739 ); goat polyclonal anti-Gal8 antibody (1:400, R&D Systems, AF1305, RRID: AB_2137229 ); rabbit polyclonal anti-KPNA3 (IMA4) antibody (1:1,000, Abclonal, A8347, RRID: AB_2770124 ); rabbit polyclonal anti-NUP107 antibody (1:1,000, Abclonal, A13110, RRID: AB_2759959 ); mouse monoclonal anti-IRS2 antibody (1:300, R&D Systems, MAB6347, 676415, RRID: AB_10992928 ); mouse monoclonal anti-FLAG M2 antibody (1:10,000, Sigma, F3165, RRID: AB_259529 ); mouse monoclonal anti-β-tubulin antibody (1:2000, AbLab, 21-0018-00, clone BT7R); mouse monoclonal anti-β-actin antibody (1:1,000, Abcam, ab8226, RRID: AB_306371 ); rabbit monoclonal anti-β-actin antibody (1:200, Abcam, ab115777, RRID: AB_10899528 ).

Techniques: Virus, Recombinant, Sequencing, Fluorescence, Modification, Protease Inhibitor, Polymer, Staining, Blocking Assay, Binding Assay, Activity Assay, Western Blot, Synthesized, Software, Control, Targeted Proteomics, Microscopy, Spectrophotometry, Mass Spectrometry

Journal: C

Article Title: Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli

doi: 10.3390/c4020029

Figure Lengend Snippet: Capture of targeted inactivated E. coli -GFP bacteria on OSX and polydimethylsiloxane (PDMS) polymers imprinted with the same inactivated bacterium. Selectivity ratios based on capture of non-targeted inactivated S. typhimurium . Total captured is based on number of targeted or non-targeted bacterial cells in 10 view fields. Selectivity was determined with S. typhimurium as the non-targeted bacterium ( n = 2).

Article Snippet: E. coli -GFP (25922GFP) and S. typhimurium -GFP (subspecies enterica serovar Typhimurium GFP, 14028GFP) were purchased from ATCC (Manassas, VA, USA).

Techniques: Bacteria

Journal: C

Article Title: Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli

doi: 10.3390/c4020029

Figure Lengend Snippet: Scanning electron microscopy (SEM) images of ( A ) imprinted OSX-B polymer prepared with heat-fixed E. coli -GFP (OD 600 1.0 suspension) on polystyrene substrate, ( B ) glutaraldehyde-inactivated E. coli -GFP on OSX-C polymer prepared with E. coli -GFP (OD 600 0.44 suspension) on Thermanox, and ( C ) bright-field image of glutaraldehyde-inactivated E. coli -GFP template at 40X magnification.

Article Snippet: E. coli -GFP (25922GFP) and S. typhimurium -GFP (subspecies enterica serovar Typhimurium GFP, 14028GFP) were purchased from ATCC (Manassas, VA, USA).

Techniques: Electron Microscopy, Polymer, Suspension

Journal: C

Article Title: Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli

doi: 10.3390/c4020029

Figure Lengend Snippet: Fluorescent images of captured ( A ) glutaraldehyde-inactivated E. coli -GFP (OD 600 0.38 capture solution) and ( B ) glutaraldehyde-inactivated S. typhimurium -GFP (OD 600 0.38 capture solution) on a glutaraldehyde-inactivated E. coli -GFP-imprinted OSX-C polymer.

Article Snippet: E. coli -GFP (25922GFP) and S. typhimurium -GFP (subspecies enterica serovar Typhimurium GFP, 14028GFP) were purchased from ATCC (Manassas, VA, USA).

Techniques: Polymer

Journal: C

Article Title: Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli

doi: 10.3390/c4020029

Figure Lengend Snippet: Capture of live E. coli -GFP (OD 600 0.38 suspension) on a glutaraldehyde-inactivated E. coli -GFP-imprinted OSX-C polymer (R = 1.32; cells captured 7 ± 2; green bar) and PDMS (cells captured 20 ± 4; purple bar), n = 2.

Article Snippet: E. coli -GFP (25922GFP) and S. typhimurium -GFP (subspecies enterica serovar Typhimurium GFP, 14028GFP) were purchased from ATCC (Manassas, VA, USA).

Techniques: Suspension, Polymer

Journal: C

Article Title: Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli

doi: 10.3390/c4020029

Figure Lengend Snippet: Negative ion mode desorption electrospray (DESI) mass spectra obtained from ( A ) imprinted OSX-C polymer (unused) and ( B ) glutaraldehyde-inactivated E. coli -GFP on Thermanox (template). 1 : palmitic acid, 2 : stearic acid, b: background. Inset: schematic diagram of DESI-MS setup.

Article Snippet: E. coli -GFP (25922GFP) and S. typhimurium -GFP (subspecies enterica serovar Typhimurium GFP, 14028GFP) were purchased from ATCC (Manassas, VA, USA).

Techniques: Polymer

Journal: C

Article Title: Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli

doi: 10.3390/c4020029

Figure Lengend Snippet: Capture of targeted glutaraldehyde-inactivated E. coli -GFP (~6 × 10 5 cells/mL) on imprinted inactivated E. coli -GFP OSX-C polymer (cells captured 49 ± 9; green bar) and PDMS (22 ± 4), n = 1. Imprinting was done with template prepared from a glutaraldehyde-inactivated bacterial suspension (OD 600 2.0).

Article Snippet: E. coli -GFP (25922GFP) and S. typhimurium -GFP (subspecies enterica serovar Typhimurium GFP, 14028GFP) were purchased from ATCC (Manassas, VA, USA).

Techniques: Polymer, Suspension

Journal: Biology

Article Title: Assessing Antimicrobial Efficacy on Plastics and Other Non-Porous Surfaces: A Closer Look at Studies Using the ISO 22196:2011 Standard

doi: 10.3390/biology13010059

Figure Lengend Snippet: Literature on the different reported results of studies using the ISO 22196 standard.

Article Snippet: Healthcare settings (HVAC aluminium ducts) , Legionella pneumophila ATCC 33152 S. aureus ATCC 6538 Pseudomonas aeruginosa ATCC 15422 E. coli ATCC 8739 Candida albicans ATCC 10231 Aspergillus niger ATCC6275) , Culture medium , Tryptic soy agar was used to determine viable bacteria instead of plate count agar , Germicidal effect (ULOG 10 ) , [ ] .

Techniques: Modification, Bacteria, Incubation, Suspension, Sterility, Saline, Activity Assay, Polymer, Concentration Assay, Sonication, Control